Practical B5: Plant tissue
Investigate the process of osmosisThe process by which water moves in and out of cells from a high concentration to a low concentration. by measuring the change in length or mass of plant tissue
Procedure
- Set up 5 labelled beakers, each containing a different concentration of sucrose.
- Using a cork borer cut five potato cylinders of equal length and width.
- Weigh/measure the length of each potato cylinder.
- Add 1 potato cylinder to each beaker.
- Leave the beakers for 1 hour.
- Pat the potato cylinders dry to remove excess water.
- Reweigh/measure the length of each potato cylinder.
- Record the results in a table.
- Calculate the change in mass/length of each cylinder.
- Calculate the % change in mass/length of each cylinder 鈥 as all starting measurements may not have been the same.
- Draw a graph of % change in mass/length against the different concentrations of sucrose.
- Draw a line of best fit on the graph.
The percentage change in mass/length is calculated using the following equation:
\(\% {change} = \frac{change}{initial} {x} {100}\)
Results
The potato cylinders placed in pure water or weak sucrose solutions will gain mass/length as water will have moved from an area of high concentration (outside the potato cells) to an area of lower concentration (inside the potato cells).
The potato cylinders placed in strong sucrose solutions will lose mass/length as water will have moved from an area of high concentration (inside the potato cells) to an area of lower concentration (outside the potato cells).
The sucrose concentration that causes no change in mass/length is the concentration that is equal to the potato cell.